Coding

Part:BBa_K2960005:Design

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:12, 21 October 2019 by Aemetzloff (Talk | contribs) (→‎References)

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mlrE enzyme with TorA tat Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct was designed to be RFC[10] compatible.


Source

Sphingopyxis sp. genomic sequence. Jin H, Nishizawa T, Guo Y, Nishizawa A, Park H-D, Kato H, Tsuji K, Harada K-I. 2018. Complete genome sequence of a microcystin-degrading bacterium, Sphingosinicella microcystinivorans strain B-9. Microbiol Resour Announc 7:e00898-18. https://doi.org/10.1128/MRA.00898-18

References

Lee, P. A., Tullman-Ercek, D., & Georgiou, G. (2006). The bacterial twin-arginine translocation pathway. Annual review of microbiology, 60, 373–395. doi:10.1146/annurev.micro.60.080805.142212

Wang, J., Wang, C., Li, J., Bai, P., Li, Q., Shen, M., … Zhao, J. (2018). Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp. THN1. Frontiers in Microbiology, 9. doi: 10.3389/fmicb.2018.02238