Coding

Part:BBa_K2960002:Design

Designed by: Michelle Croen   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:10, 21 October 2019 by Aemetzloff (Talk | contribs) (→‎References)

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mlrB with TorA tat Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1297


Design Notes

We have designed this part to be RFC[10] compatible. We also optimized codons for expression in E. coli.


Source

European Nucleotide Archive. (2015, April 16). Retrieved from https://www.ebi.ac.uk/ena/data/view/KR150744.


References

Lee, P. A., Tullman-Ercek, D., & Georgiou, G. (2006). The bacterial twin-arginine translocation pathway. Annual review of microbiology, 60, 373–395. doi:10.1146/annurev.micro.60.080805.142212

Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187