Protein_Domain
Part:BBa_K103009
Designed by: Michael Lower Group: iGEM08_Warsaw (2008-10-09)
linker fused to alpha fragment of TEM beta-lactamase
Usage and Biology
- alpha fragment of the split enzyme TEM beta-lactamase
- beta lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
- works with second half of beta lactamase (part BBa_K103013)
- contains linker module that allows proper binding of fusion partners
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
//function/reporter
//function/reporter
protein |