Coding
Part:BBa_K2960001:Design
Designed by: Michelle Croen Group: iGEM19_Cornell (2019-10-16)
mlrA with TorA tat Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 437
Illegal AgeI site found at 566 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The construct was designed to be RFC[10] compatible.
Source
European Nucleotide Archive. (2008, October 22). Retrieved from https://www.ebi.ac.uk/ena/data/view/BAI47770.
References
Lee, P. A., Tullman-Ercek, D., & Georgiou, G. (2006). The bacterial twin-arginine translocation pathway. Annual review of microbiology, 60, 373–395. doi:10.1146/annurev.micro.60.080805.142212