Coding
AgrA2
Part:BBa_K3183004:Design
Designed by: David Schramm Group: iGEM19_Oxford (2019-10-16)
AgrA2 Quorum Sensing Transcription Factor; C. difficile
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for L. reuteri using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]
RF10, 12, 21, 23, 25, and 1000 Compatible
Source
Derived from the C. difficile strain CD196 Genome; synthesized in IDT gBlocks.
References
1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.