Composite

Part:BBa_K3286205

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)
Revision as of 23:50, 20 October 2019 by Benkuipers (Talk | contribs)


Vc2 Riboswitch + sfGFP

This composite part contains the Vc2 riboswitch (BBa_K3286202), which was used in combination with composite partBBa_K3286204. The function of this part is that upon DSF sensing from part BBa_K3286204, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 Riboswitch, which will result in sfGFP (BBa_K3286203) translation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None