Part:BBa_K3286202

Vc2 Riboswitch

This is the regulatory sequence of the Vc2 riboswitch. The Vc2 riboswitch is a class I c-di-GMP riboswitch which acts on the translational level. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression and translation of the gene will occur [1,2].

Vc2 riboswitches are regulatory elements naturally occurring in Vibrio cholerea. Although, class I riboswitches in general are phylogenetically widespread among the bacterial kingdom [3].

1. S. Inuzuka et al., “Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism,” J. Biochem., vol. 160, no. 3, pp. 153–162, 2016.
2. S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.
3. N. Sudarsan et al., “Riboswitches in Eubacteria Sense the Second Messenger Cyclic Di-GMP,” Science (80-. )., vol. 321, no. 5887, pp. 411–413, Jul. 2008.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]