Translational_Unit

Part:BBa_K3165030

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)
Revision as of 22:58, 20 October 2019 by Souvadra (Talk | contribs) (→‎Usage and Biology)

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RBS + Ho1

Heme Oxygenase 1 (Ho1) coding sequence with a ribosome binding site (RBS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Biology

The heme oxygenase coding sequence (encoding the enzyme for the oxidative degradation of heme into biliverdin IX alpha) is coupled with an RBS (BBa_B0034) , resulting in the formation of a translational unit. The RBS (with a Shine Dalgarno like sequence) helps in the binding of the ribosome initiating the process of translation.

Usage

This part is used to translate the ho1 gene construct into the functional enzyme under a suitable promoter. In association with the translational unit coding for PcyA (BBa_K3165058), this sequence can act as a genetic circuit for the biosynthesis of phycocyanobilins (PCBs).


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Categories
Parameters
None