Part:BBa_K3202035:Design
TetR-Pc-Ptet-Bxb1
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2112
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1838
Illegal XhoI site found at 1751 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1112
Illegal NgoMIV site found at 1199
Illegal AgeI site found at 2062 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1004
Design Notes
NA
Source
Recombinase cds given by GENAS_China, synthesized
References
【1】 LWOFF, A. Lysogeny. Bacteriol. Rev. 17, 269–337 (1953) 【2】Campbell A. (2006) in The Bacteriophages, General aspects of lysogeny, ed Calendar R. (Oxford University Press, Oxford), 2nd edn, pp 66–73. 【3】Myers, C. J. Engineering Genetic Circuits. 306 (CRC Press, 2009) 【5】Azaro M.A., Landy A. (2002) in Mobile DNA II, λ integrase and the λ Int family, eds Craig N.L., Craigie R., Gellert M., Lambowitz A.M. (ASM Press, Washington, DC), pp 118–148. 【6】Yueju Wang. Recombinase technology: applications and possibilities. Plant Cell Rep. 2011 Mar; 30(3): 267–285. 【7】Gretchen Meinke. Cre Recombinase and Other Tyrosine Recombinases. Chem. Rev., 2016, 116 (20), pp 12785–12820. 【8】Grindley N D F, Whiteson K L, Rice P A. Mechanisms of site-specific recombination. Annu Rev Biochem, 2006, 75: 567—605 【9】Hsu P L, Ross W, Landy A. The λ-phage att site: functional limits and interaction with Int protein. Nature, 1980, 285: 85—91 【10】Abremski K, Gottesman S. Site-specific recombination: Xis-independent excisive recombination of bacteriophage λ. J Mol Biol, 1981, 153: 67—78 【11】Abremski K, Hoess R. Bacteriophage P1 site-specific recombination: purification and properties of the Cre recombinase protein. J Biol Chem, 1984, 259: 1509—1514 【12】Decapentaplegic and growth control in the developing Drosophila wing. Takuya Akiyama & Matthew C. Gibson. Nature 527, 375–378 (19 November 2015) doi:10.1038/nature15730 【13】Alexandra Pokhilko et al. The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling. Nucleic Acids Research, pp. gkw616, 2016, ISSN 0305-1048. 【14】Nathaniel Roquet1, Ava P. Soleimany, Alyssa C. Ferris, Scott Aaronson, Timothy K. Lu. Synthetic recombinase-based state machines in living cells. Science 22 Jul 2016: Vol. 353, Issue 6297 【16】Benjamin H Weinberg et al. Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells. Nature Biotechnology 35, 453–462 (2017) doi:10.1038/nbt.3805 【17】Jesus Fernandez-Rodriguez，Lei Yang，Thomas E. Gorochowski，D. Benjamin Gordon，Christopher A. Voigt. Memory and Combinatorial Logic Based on DNA Inversions: Dynamics and Evolutionary Stability. ACS Synth. Biol., 2015, 4 (12), pp 1361–1372.