![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K3292005:Design
Designed by: Gerardo David Díaz Renovato, Abril Diosdado Hernández Group: iGEM19_Tec-Monterrey (2019-10-20)
DNA synthesis regulated promoter (yorB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 120
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design considerations already appear in the description
Source
We obtained the sequence of this part from the NCBI genome database using the primers provided by Urban et al. (2007)
References
Hutter, B., Fischer, C., Jacobi, A., Schaab, C., & Loferer, H. (2004). Panel of Bacillus subtilis reporter strains indicative of various modes of action. Antimicrobial agents and chemotherapy, 48(7), 2588–2594. doi:10.1128/AAC.48.7.2588-2594.2004
Urban, A., Eckermann, S., Fast, B., Metzger, S., Gehling, M., Ziegelbauer, K., Rübsamen-Waigmann, H., Freiberg, C. (2007). Novel whole-cell antibiotic biosensors for compound discovery. Applied and environmental microbiology, 73(20), 6436–6443. doi:10.1128/AEM.00586-07