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Part:BBa_K3009012

Designed by: Carolin Ruckes   Group: iGEM19_Freiburg   (2019-10-15)
Revision as of 21:01, 20 October 2019 by Caroruckes (Talk | contribs)

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Aspartate Racemase (PhAspR)

Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. The aspartate racemase is consequently converting L-aspartate to D-aspartate.


We purified it via his-tag purification to perform further experiments.

Fig.2: His-Tag Purification of P.horikoshii aspartate racemase. Bands are clearly visible at 40 kDA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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