Part:BBa_K3286138
flg22
Flg22 is a peptide consisting of 22 conserved amino acids at the N-terminus flaggelin of Pseudomonas syringae [Felix et al., 1999]. Flg22 is a well described Microbe Associated Molecular Pattern (MAMPs). If recognised, MAMPS trigger PAMP Triggered Immunity in plants. They have been shown to trigger disease resistance in plants. [Zipfel et al., 2004]. flg22 can be used to active an immune response in plants.
It is very hard to purify this peptide if recombinantly expressed in E. coli</en>, due to the small size and the folding of this peptide.
Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 58
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 58
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 58
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 58
Illegal NgoMIV site found at 52
Illegal AgeI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
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