Plasmid

Part:BBa_K3179105:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)
Revision as of 20:40, 20 October 2019 by Bigxushen (Talk | contribs) (→‎Design Notes)


pT2mKE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 999
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 999
    Illegal NgoMIV site found at 473
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists of miR663b-target, 2kt-E1A and miR885-5p-target,which be put in the vector pTRE. From promoter CMV our plasmid can transcribe mRNA with miR663b-target, 2kt-E1A and miR885-5p-target, whose translation can be regulated by level of free miRNA663b and miRNA885-5p, and also by L7Ae. When they exist, the translation of this mRNA will be suppressed. The production is E1A, which can start the expression of adenoviral gene.

Source

pTRE-mi663b-2Kt-E1-miR885-5p

References

1 Wroblewska L, Kitada T, Endo K, et al. Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery[J]. Nature biotechnology, 2015, 33(8): 839. 2 Berk A J. Functions of adenovirus E1A[J]. Cancer surveys, 1986, 5(2): 367-387.