Coding

Part:BBa_K3320013

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)
Revision as of 20:40, 20 October 2019 by Jocomodaro (Talk | contribs)


GFPmut3 + HlyA

This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system.

Usage and Biology

GFP is a fluorescent protein from Aequorea victoria. This protein is fusioned with the HlyA secretion tag from the alpha-hemolysin system of E. coli, It allows the GFP to be secreted directly from the cytosol to the extracellular medium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None