Regulatory

Part:BBa_K2978206

Designed by: Jose Pablo Delgado Navarro   Group: iGEM19_Costa_Rica   (2019-10-19)
Revision as of 20:31, 20 October 2019 by RyuNeon17 (Talk | contribs)


Lactobacillus spp Slp constitutive promoter

This part refers to the Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

  • The sequence was the same used in the addgene plasmid pTRKH3-slpGFP. It is reported that this promoter expresses a high level of transcripts of mRNA, and it is proved that works in different strains of Lactobacillus spp.
  • This same promoter was used by Lubkowicz and colleagues in 2018 while developing a biosensor for Staphylococcus aureus infection.

References

  • Lubkowicz, D., Ho, C. L., Hwang, I. Y., Yew, W. S., Lee, Y. S., & Chang, M. W. (2018). Reprogramming probiotic Lactobacillus reuteri as a biosensor for Staphylococcus aureus derived AIP-I detection. ACS synthetic biology, 7(5), 1229-1237.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None