Part:BBa_K3292005
DNA synthesis regulated promoter (yorB)
This promoter regulates the expression of the YorB protein in B. subtilis subtilis str. 168 and was initally reported by Hutter et al. (2004) and further characterized by Urban et al. (2007) for its induction by the antibiotic interference of DNA biosynthesis in B. subtilis.
As part of our project, we flanked the core sequence with sites of type IIs restriction enzymes, particularly BsaI and BsmBI (also called for its isoschizomer's name Esp3I), to allow its usage with Golden Gate cloning. Finally, we added Biobrick prefix and suffix to make it compatible with RFC 10 standard, and M13 forward and reverse primer sequences to enable the PCR amplification of this parts as gBlocks.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 120
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |