DNA

Part:BBa_K2940003

Designed by: Francisco Ivan Rodriguez Jaubert   Group: iGEM19_Edinburgh_OG   (2019-10-15)
Revision as of 18:45, 20 October 2019 by Ivanjaubert (Talk | contribs)


Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal SpeI site found at 128
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 128
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None