Part:BBa_K3059635
Constitutive Curli Operon: J23107
Synthetic curli operon under medium-weak constitutive promoter J23107. Curli fiber expression was assessed via Congo Red spin-down assays, where any present fibers bind Congo Red dye and hold it in the cell pellet, resulting in a lighter (less red) supernatant. Congo Red results are quantified by measuring A490 of this supernatant.
For this circuit (WM19_024 or "circuit 24"), so visible pellets formed in the glass culture tubes during overnight growth, and no difference in redness was observed between control and experimental pellets after Congo Red staining. Furthermore, the average A490 measurement for experimental samples’ supernatants was slightly higher than the average for control circuits (if curli fibers were expressed, the supernatant should be lighter, resulting in a lower A490 measurement). Future constitutive circuits may require a stronger promoter such as J23100.
More detailed characterization (as well as photos) is available on our wiki.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1873
Illegal PstI site found at 972 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1873
Illegal NheI site found at 51
Illegal NheI site found at 74
Illegal PstI site found at 972 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1873
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1873
Illegal PstI site found at 972 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1873
Illegal PstI site found at 972
Illegal AgeI site found at 2170 - 1000COMPATIBLE WITH RFC[1000]
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