Composite

Part:BBa_K3202053:Design

Designed by: Yiming Dong   Group: iGEM19_BHSF_ND   (2019-10-16)
Revision as of 18:15, 20 October 2019 by Katherine Z4 (Talk | contribs)


AraC-Pc-pBAD-Kid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This part is designed mainly for the characterization of the toxin parE. In our study, we ought to ensure the toxin encoded within the bistabe system is efficient for killing any bacteria. Therefore, by presenting this cThis part is designed mainly for the characterization of the toxin parE.omposite part with BBa_K3202053 and BBa_K3202054, we provide a tool pack aiming different kinds of bacteria.


Source

Synthesis

References

Reference: [1]:A RelE/ParE superfamily toxin in Vibrio parahaemolyticus has DNA nicking endonuclease activit Biochemical and Biophysical Research Communications, 15 July 2017, Pages 29-34 [2]:Structural and Functional Analysis of the Kid Toxin Protein from E. coli Plasmid R1. Structure, Volume 10, Issue 10, October 2002, Pages 1425-1433