Composite

Part:BBa_K3147005:Design

Designed by: Thomas Bessede   Group: iGEM19_Montpellier   (2019-10-13)
Revision as of 16:35, 20 October 2019 by ThomasBCD (Talk | contribs) (→‎Design Note)

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TEV protease with TEV-cleavable maltose binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Note

The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.

The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G Part:BBa_ J18918 with \ indicating cleaved peptide binding.

The Terminator used is a double terminator Part:BBa_B0015.