Composite

Part:BBa_K3165056

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)
Revision as of 16:33, 20 October 2019 by Registry (Talk | contribs)

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N-T7 - nMag + C-T7 - pMag + Gp2 (for Escherichia coli)

This composite system consists of the entire optogenetic T7 RNA Polymerase system with Gp2 under the pCpcG2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2367
    Illegal NheI site found at 2390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1678
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3800
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1350
    Illegal BsaI.rc site found at 2874


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Parameters
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