Part:BBa_K3286043

Native bacteriophage Lambda PR and PRM promoter, OR operators and 5' UTR

The native bacteriophage Lambda PR and PRM promoter of bacteriophage Lambda, with the operator regions OR1, OR2 and OR3, and the PR 5'UTR.

The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.

References

Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]