Measurement

Part:BBa_K2972008

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-16)
Revision as of 14:57, 20 October 2019 by UOH4 (Talk | contribs)


Quorum sensing(QS) sensitivity test

Introduction

This part is used to test the sensitivity of QS system. To discover the relationship between the components produced by the QS system and the density of cells in the process of cell growth.

When considering the change of signal factor concentration in a single cell, we refer to the model of Maike in 2010. Because our model is in a closed container, we delete the diffusion term from the original model, and improve the solvability. We modified and established our own model according to the actual situation.

BIT-China Modeling1.jpg BIT-China Modeling2.jpg

Result

In this result, we can clearly see that AHL showed very low growth rate in the earlystage. At a certain time, when the population density reached a certain threshold,AHL showed a sharp increase trend, indicating that quorum sensing has occurred. The growth rate of LuxI also began to increase suddenly, and a large number of products were produced. This result shows that our model is very consistent with the phenomenon of quorum sensing, and can be used as a mathematical description of the cell growth state in the experiment. This model can be great significance to the experimental guidance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1634
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Categories
Parameters
None