Part:BBa_K3182010

Parseq-β

Parseq Beta is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD₆₀₀ was measured. The results can be seen in Figure 1..Parseq Beta showed an inhibiting effect on the growth of E.coli. A concentration of 100 µg/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Beta. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.

Figure 1. Shows the antimicrobial activity of Parseq Beta on A) E.Coli. and B) B.subtilis. The samples were cultured in 200 µL, in a 96 well plate and incubated at 37°C. Shaking was conducted for 1 minute every 15 minutes and the OD₆₀₀ was measured every hour for 16 hours. The negative control represents the bacterial growth with no antimicrobial peptides present. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]