Composite

Part:BBa_K3064005

Designed by: Yating Long   Group: iGEM19_NUDT_CHINA   (2019-09-24)
Revision as of 13:23, 20 October 2019 by Xusu (Talk | contribs) (→‎Usage and Biology)

3XGlucose Sensing Promter

This composite part is a kind of promoter which is sensitive to particular blood glucose concentration.

Usage and Biology

Because there is high blood glucose concentration in diabetics, in order to get improved gene which is sensitive to diabetics it’s convenient to employ promoter which reacts to high blood glucose. Transcription factor -- ChREBP -- can be effectively expressed with high blood glucose. Meanwhile, heterodimer which consists of ChREBP and Mlx can combine with gene promoter ChoRE to induce gene transcription¹.Therefore, we choose ChoRE as promoter of engineered plasmid and connect it with minP to indicates a transcription start site. In addition, we increase the number of ChoRE to realize that engineered gene will be activated by particular high blood glucose concentration instead of normal blood glucose concentration.

In this composite part, we used one minP and three ChoRE and apply luciferase gene as downstream reporter in plasmid PGL3-3XGSP. conducted experience to figure out the specific blood glucose concentration which can activate this composite part.

References

[1] Li Ma,PengFei Gao,JianZhong Shi,et al.Research progress of ChREBP[J].Animal Husbandry and Feed Science,2014,35(09):40-42(Chinese)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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