Composite

Part:BBa_K2922043:Design

Designed by: Yuan Li   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 12:55, 20 October 2019 by Xv-Jiang (Talk | contribs)


Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 328
    Illegal NotI site found at 1472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1385
    Illegal BamHI site found at 521
    Illegal XhoI site found at 883
    Illegal XhoI site found at 1132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 1588
    Illegal NgoMIV site found at 2906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 567


Design notes

We ordered the part DNA from Sangon ® company.


Source

DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.