RNA

Part:BBa_K3171182

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-20)
Revision as of 12:53, 20 October 2019 by Registry (Talk | contribs)

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HisD gRNA 3

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. Specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) and 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) which all fall under the category of basic parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None