Composite
IBR-G22

Part:BBa_K3254005

Designed by: Yeqing Zong, YI REN   Group: iGEM19_GENAS_China   (2019-10-13)
Revision as of 12:15, 20 October 2019 by Zongyeqing (Talk | contribs)


TG1attB-BsaI site-terminator-TG1attP

We expected that this part can be placed between a promoter and a translational unit part and work as a normally open (NO) switch for the downstreamed gene. and switch to ON state by excising the terminator L3S3P22 between the att sites when it reacted with TG1 integrase (BBa_K2460007). Two BsaI restriction site were added between attB site and terminator. As a result, it can work as a normally closed (NC) switch for the gene which was inserted between the two BsaI site and switch to OFF state when it was excised.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 48


[edit]
Categories
//function/recombination
Parameters
None