Reporter

Part:BBa_K121012

Designed by: Masashi Kajita   Group: iGEM08_Tokyo_Tech   (2008-10-16)
Revision as of 15:11, 22 October 2008 by Kiga (Talk | contribs)

Plac GFP on pSB6

pressure and IPTG inducible Plac GFP reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954

Usage and Biology

Identify Hill coefficient by IPTG induction

Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.

Transfer Function K121012.png

The hill coefficient is 2.15.

Pressure induction




[edit]
Categories
Parameters
None