Part:BBa_K2983051

pTef1d: wild-type without the BsaI site

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227374 to 1226969) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1).

It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Usage and Biology

TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α [1]and is also widely used to drive constitutive gene expression in Y. lipolytica.[2]

References

[1] Müller S, Sandal T, Kamp-Hansen P, Dalbøge H. Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast. 1998 Oct;14(14):1267-83. PMID: 9802206 [2]Trassaert, M. et al. New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica. Microb Cell Fact 16, 141 (2017).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 199
  Illegal PstI site found at 164
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 199
  Illegal PstI site found at 164
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 199
  Illegal PstI site found at 164
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 199
  Illegal PstI site found at 164
  
• 1000
  COMPATIBLE WITH RFC[1000]