Reporter

Part:BBa_K121012

Designed by: Masashi Kajita   Group: iGEM08_Tokyo_Tech   (2008-10-16)
Revision as of 14:37, 22 October 2008 by Ryo (Talk | contribs)

Plac GFP on pSB6

pressure and IPTG inducible Plac GFP reporter

Usage and Biology

Identify Hill coefficient by IPTG induction

Plac is known to be induced by IPTG. Using iGEM2007 Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in

E. Coli strain JM109.

Transfer Function K121012.jpg

The hill coefficient is 2.15.

Pressure induction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954


[edit]
Categories
Parameters
None