Regulatory

Part:BBa_K2983050

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 11:42, 20 October 2019 by Anthor91 (Talk | contribs) (→‎Usage and Biology)


pTef1c: BBa_K2117000 without the BsaI site

This part is a version of the Yarrowia lipolytica pTef1 promoter. It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Usage and Biology

TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α [1]and is also widely used to drive constitutive gene expression in Y. lipolytica.[2]

References

[1] Müller S, Sandal T, Kamp-Hansen P, Dalbøge H. Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast. 1998 Oct;14(14):1267-83. PMID: 9802206 [2]Trassaert, M. et al. New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica. Microb Cell Fact 16, 141 (2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None