Coding

Part:BBa_K3113051

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-09-05)
Revision as of 11:42, 20 October 2019 by Theresakeil (Talk | contribs)


CD63_Ser161_6xHis

To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin. Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.

6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org

<figure class="figure"> <img src="T--Munich--Improved_CD63.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> </figure>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None