Regulatory

Part:BBa_K2983053

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 11:32, 20 October 2019 by Anthor91 (Talk | contribs)


pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.

It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Usage and Biology

TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None