Regulatory
Part:BBa_K2983052
Designed by: Tom Zaplana Group: iGEM19_Evry_Paris-Saclay (2019-10-12)
pTef1e: wild-type without the BsaI site and shorter
This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the Addgene plasmid #120738.
It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
Usage and Biology
TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |