Composite

Part:BBa_K2963014

Designed by: Experiment group advisor of JNU-China   Group: iGEM19_JNU-China   (2019-09-10)
Revision as of 11:12, 20 October 2019 by Chikaijun (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


BsracE18 - Bicistronic containing RBS18 to stabilize gene expression.


Usage and Biology

The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the racE gene, we wanted to stabilize the expression of racE gene by using bicistronic system.

Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
    Illegal NheI site found at 999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None