Generator

Part:BBa_K3165049

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)
Revision as of 10:54, 20 October 2019 by Registry (Talk | contribs)

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i^2mCherry (Leu) Coding Device (under T7 expression)

This part is used to generate i2mCherry (Leu) which overcomes the problems in the existing mCherry sequence which undergoes significant truncation in the protein. The 2 single-base mutations at the 46th and 48th base causes conversion of the internal start codon into CTT (coding for Leu).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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