Part:BBa_K2996011

RSRL-eGFP downstream of pTac

The ribosome-binding site and the translation initiation codon of this reading device are indicated. Translation of transcripts generated from the tac promoter will stop at the leader, for those two stop codons. The wrong reading frame and existing stop codon together will insure no expression of the florescent protein in the original situation.

File:Figurefilename.png

Schematic representation of the integration cassettes RSRL array cloned in the pRead plasmid and strategy used for specific detection of integration events.

Upon information input, that is addition of corresponding inducer, spacer adaptation from cas1/2 will result in an addition of 61 base pairs, 33bp new spacer and 28bp replicated repeat, into the integration cassette. Stop codon will be moved out of frame, while EGFP in the open reading frame. Thus, successful translation of EGFP is our desired information output signal, observed by eyes and microplate reader.

Result

tet做诱导剂的四种 Eye PCR Microplate reader Sequencing

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 252
  Illegal BamHI site found at 978
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]