Regulatory
P-erm

Part:BBa_K3183000:Design

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-09-20)
Revision as of 10:44, 20 October 2019 by Dschramm (Talk | contribs) (→‎Source)


Erythromycin Constitutive Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards.

Source

It is the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis. We ordered the sequence as a gBlock from IDT.

References