Composite

Part:BBa_K3196025

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
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AOX1-Kozak-SUC2-VP-His tag-AOX1 Terminator

The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.The expression of SUC2 gene is a kind of Sucrose transferase [1], and its signal peptide can help the protein out cells. We use this signal peptide raise the efficiency of VP’s transformation.
Figure1. T--HUST--China--2019-SUC2VP


Reference

[1]Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. Nooshin Mohandesi, Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour Received: 8 February 2016 / Accepted: 25 May 2016 / Published online: 8 June 2016 The Author(s) 2016.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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