Coding
Part:BBa_K3286207
Designed by: Ben Kuipers Group: iGEM19_Wageningen_UR (2019-10-20)
RpfCch (TMD) sfGFP fusion
This part is similar to part BBa_K3286206, but in this strategy, only the transmembrane domain (TMD)was used to fuse with sfGFP. So again, this part was constructed to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |