![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2913021
Designed by: Yannan Wang Group: iGEM19_NEFU_China (2019-10-20)
Cytosine deaminase
An enzyme that oxidizes 5-fluorouracil to 5-fluorouracil
Usage and Biology
result
Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil 5-FU, which has an extreme damage to the bacteria.
We added gradient of 5-FC culturing the bacteria and monitor their growth.
![](/wiki/images/b/bc/T--NEFU_China--parts--CD1.png)
Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.
As a positive control, we added gradient of 5-FU culturing the bacteria and monitor their growth.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1140
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |