Generator

Part:BBa_K3165046

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)
Revision as of 09:49, 20 October 2019 by Mohitdas (Talk | contribs)


i^2mCherry (Ile) Coding Device (under T7 expression)

i^2mCherry overcomes the deficiencies in the existing mCherry sequences which undergoes significant truncation in the protein. The single-base mutation at the 48th base causes conversion of the internal start codon into ATC (coding for Ile). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

[edit]
Categories
Parameters
None