Reporter

Part:BBa_K3286203

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)
Revision as of 09:33, 20 October 2019 by Benkuipers (Talk | contribs)


GFP superfolder

This is the coding sequence of a super folder green fluorescent protein (sfGFP). sfGFP have been described to be better suitable reporters than other GFP (mutated) versions[1].

[1] J. D. Pédelacq, S. Cabantous, T. Tran, T. C. Terwilliger, and G. S. Waldo, “Engineering and characterization of a superfolder green fluorescent protein,” Nat. Biotechnol., vol. 24, no. 1, pp. 79–88, 2006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None