Part:BBa_K3245002

BBa_K3245002:

Usage and biology:

In QS system, the upstream product gets higher concentration as the population grows, which then regulates activity of the downstream promotor. In this part, LuxR activator protein combines with homoserine lactone(HSL) produced by LuxI protein as dimers. The dimers then up-regulate the expression of luxpr.

Design:

QS system is the main regulating circuit in our project. After comparing the efficiency of different QS system，we chose LuxI and LuxR to regulate the downstream circuit. To balance and stabilize the copy number of luxI (BBa_C0061) and luxR(BBa_C0062), we designed this plasmid carrying the two gene at the same time. Both gene uses the J23106 promoter and B0015 terminator. As a upstreaming plasmid for inducing, its measurement needs to cooperate with another downstream QS promotor (luxpr-GFP in our experiment).

Characterization:

For more information: click https://parts.igem.org/Part:BBa_R0062 to see characterization of BBa_R0026

Fig,1 this figure shows the WT luxpr expression level induced by luxR and luxI. The MEFL/particle changes as the OD goes up after dilution.

Final dimeric production regulating luxpr

In QS system, the upstream product gets higher concentration as the population grows, which then regulates activity of the downstream promotor. In this part, LuxR activator protein combines with homoserine lactone(HSL) produced by LuxI protein as dimers. The dimers then up-regulate the expression of luxpr.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 678
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]