Regulatory

Part:BBa_K112407

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-21)
Revision as of 03:27, 22 October 2008 by ChrisBrown (Talk | contribs)

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Promoter for ygeF psuedogene

Our microarray results showed that this gene was upregulated four fold in response to low frequency ultrasound and two fold in response to audible sound. We cloned approximately 400 base pairs upstream from the start codon for the ygeF pseudogene.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. In a Bca1256 plasmid backbone. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/unknown
Parameters
negative_regulators-1
positive_regulators-1