Composite

Part:BBa_K3126021

Designed by: Qianxin Li   Group: iGEM19_HBUT-China   (2019-10-16)
Revision as of 07:59, 20 October 2019 by Lqs991122 (Talk | contribs)


hexa-His-nixA-KanR

We connected hexa-his and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part. We also compared its function with those two functional genes working separately (Part:BBa_K3126018 Part:BBa_K3126019).

Usage and Biology

Here is the curve comparing with Saccharomyces cerevisiae expressing Part:BBa_K3126018 and Part:BBa_K3126019. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with BBa_K3126018 and BBa_K3126019

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4690
    Illegal AgeI site found at 1049
    Illegal AgeI site found at 1310
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None