Composite

Part:BBa_K3196021

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 07:30, 20 October 2019 by Zyz2018hust (Talk | contribs)


AOX1-Kozak-FLO10-VP-His tag-AOX1 Terminator

FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 is a kind of signal peptide which was initially found before the protein involved in flocculation. It is supposed that the signal peptides from pichia pastoris can be better identified by its signal recognition system, thus benefiting the secretion of exogenous protein. So we add the coding sequence of FLO10 before VP to figure out whether it could increase the output of VP.

Figure1. T--HUST--China--2019-FLO10VP

DNA Gel Electrophoretic

After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None