Composite

Part:BBa_K3196015

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 07:26, 20 October 2019 by GlacierHOLE (Talk | contribs)


The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. SLAC can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. T--HUST--China--2019-SUC2SLAC



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


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Categories
Parameters
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