Part:BBa_K2997003

Sigma 54-dependent transcriptional regulator (pchR)

Background

The pchR gene is responsible for the production of activator proteins that can bind with the inducer (p-Cresol) and leads to the conformation of the protein. ^[1][2] In other words, the activator protein can bind with p-Cresol to form a protein-p-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-p-Cresol complex initiates the expression of the downstream gene, in our case GFP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1738
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1151
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1196
  Illegal AgeI site found at 179
  Illegal AgeI site found at 1079
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1105
  

References

1. Cho, Ah Ra, Eun Jin Lim, Yaligara Veeranagouda, and Kyoung Lee* Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea . Identification of a p-Cresol Degradation Pathway by a GFP-Based Transposon in Pseudomonas and Its Dominant Expression in Colonies
2. Merike Jo ˜esaar, Eeva Heinaru, Signe Viggor, Eve Vedler & Ain Heinaru Institute of Molecular and Cell Biology, Tartu University, Tartu, Estonia. Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-Cresol-degradative strains of Pseudomonas fluorescens